Molecular detection of Babesia bovis in cattle in Al-Qadisiyah province

Molecular Detection of Babesia bigemina and Babesia bovis in Crossbred Carrier Cattle Through PCR

Bovine babesiosis and other tick borne diseases are considered responsible for more than 50% losses in the crossbred cattle. Carrier cattle infected with babesiosis are challenge to current diagnostic methods and are difficult to detect because of the low number of parasites in circulation. However, diagnosis of carrier animals in herd is important for preventing outbreaks by transmission throu...

Detection of Babesia bovis in cattle by PCR-ELISA.

We established a new highly sensitive method, PCR-ELISA, for the dectection of Babesia bovis in cattle for farms in Thailand. The detection of around 2.4 x 10(-8)% parasitemia (equivalent to 1 infected erythrocyte per 2 ml) was achieved by PCR amplification followed by the ELISA detection of a biotin tagged gene. When comparing the sensitivity of PCR-ELISA with the microscopic method, our PCR-E...

Molecular Detection of Anaplasma bovis in Cattle from Central Part of Iran

Anaplasma bovis is a leukocytotropic agent of bovine anaplasmosis and there is no available information about molecular study on this agent in cattle of Iran. In this study a total 150 cattle blood samples were collected from central part of Iran. The presence of A. bovis examined using light microscopic detection and species-specific nested polymerase chain reaction (nested-PCR) based on 16S r...

An outbreak of Babesia bovis infection in cattle and its control

A molecular study on Babesia spp. in cattle and ticks in West-Azerbaijan province, Iran

A total number of 450 blood samples were collected from 45 different randomly selected cattle herds. Light microscopic examination of blood smears revealed Babesia spp. infection in 4.2%, while 8.9% of blood samples were positive using PCR. Upon multiplex-PCR (mPCR), B. bigemina and B. bovis infections were detected in 37/40 (92.5%) and 3/40 (7.5%) samples, respective...